Knockout of circRNAs by base editing back-splice sites of circularized exons

Knockout of circRNAs by base editing back-splice sites of circularized exons

Blog Article

Abstract Many circular RNAs (circRNAs) are produced from back-splicing of exons of precursor mRNAs and malibu relaxer are generally co-expressed with cognate linear RNAs.Methods for circRNA-specific knockout are lacking, largely due to sequence overlaps between forms.Here, we use base editors (BEs) for circRNA depletion.

By targeting splice sites involved in both back-splicing and canonical splicing, BEs can repress circular and linear RNAs.Targeting sites predominantly for circRNA biogenesis, BEs could efficiently repress the production of circular sm-x610nzaaxar but not linear RNAs.As hundreds of exons are predominantly back-spliced to produce circRNAs, this provides an efficient method to deplete circRNAs for functional study.